Inhalable Cationic Niosomes of Curcumin Enhanced Drug Delivery and Apoptosis in Lung Cancer Cells
1, 2, 1, 1
- 1Department of Pharmaceutics, Chandigarh College of Pharmacy, Mohali (Panjab) INDIA.
- 2Department of Pharmaceutical Biotechnology, SLT Institute of Pharmaceutical Sciences, Guru Ghasidas University, Bilaspur, INDIA.
CORRESPONDENCE
Jitender Madan
Department of Pharmaceutics, Chandigarh College of Pharmacy, Mohali (Panjab) INDIA.
Received: 08-07-2015; Accepted: 16-02-2016.
Volume 50, Issue 2s (Supplement) · pp. s21-s31 · 2016 · DOI: 10.5530/ijper.50.2.14
ABSTRACT
Background: Curcumin induces apoptosis in non-small cell lung cancer cells. Hence, inhalable cationic niosomes of curcumin were developed to surmount the poor physicochemical and biopharmaceutical limitations for effective drug delivery in lung cancer cells.Methods: Curcumin loaded freeze-dried cationic small unilamellar niosomes (Cur-C-SUNS) were prepared using reverse phase evaporation method and characterized in vitro using spectral, analytical and biological techniques.Results: The nanovesicle size, encapsulation efficiency and zeta-potential of Cur-C-SUNS were measured to be 97.4±8.3 nm, 83.3±5.1% and +28.5±1.25 mV, significantly (P<0.05) higher than 83.8±7.2 nm, 78.8±4.5% and -3.02±0.64 mV of optimized freeze-dried Cur-SUNS. Cur-C-SUNS inhibited the A549 lung cancer cells proliferation at the IC50 of 3.1 μM, significantly (P<0.05) lower than 7.5 μM of Cur-SUNS and curcumin suspension (<32 μM). Consistently, Cur-C-SUNS induced greater extent of apoptosis in comparison to Cur-SUNS and curcumin suspension. In addition, Cur-C-SUNS accumulated significantly (P<0.05) higher concentration of curcumin, 14.3±2.1 μg in A549 cells, as compared to 9.5±1.5 μg and 1.3±0.2 μg deposited, respectively by Cur-SUNS and curcumin suspension. At last, in vitro cellular uptake illustrated higher endocytosis of Cur-C-SUNS as compared to Cur-SUNS due to electrostatic interaction between cationic nanovesicles and negatively charged plasma membrane of A549 cells.Conclusion: In conclusion, promising in vitro attributes of Cur-C-SUNS in lung cancer therapy warrant further in vivo tumor regression study to scale up the technology for clinical translation.
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Jyoti, K., Pandey, R. S., Madan, J., & Jain, U. K. (2016). Inhalable Cationic Niosomes of Curcumin Enhanced Drug Delivery and Apoptosis in Lung Cancer Cells. Indian Journal of Pharmaceutical Education and Research, 50(2s), s21-s31. https://doi.org/10.5530/ijper.50.2.14
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