Chondroprotective and Anti-Inflammatory Activities of Extracts from Semen Sojae Germinatum on IL-1 β- Stimulated Human Osteoarthritis Chondrocytes
1, 1, 1, 1, 2, 2
- 1Department of Pharmacy, College of Medicine, Wuhan University of Science and Technology, Wuhan-430065, CHINA.
- 2Department of Orthopedic Surgery, Hubei Woman and Child Hospital, Wuhan-430070, CHINA.
CORRESPONDENCE
Tong Gui
Department of Orthopedic Surgery, Hubei Woman and Child Hospital, Wuhan-430070, CHINA.
Received: 17-01-2016; Accepted: 06-04-2016.
Volume 50, Issue 3 · pp. 397-402 · 2016 · DOI: 10.5530/ijper.50.3.12
ABSTRACT
Our previous study showed that Semen sojae germinatum (SSG), a soy-derived Chinese medicinal material, have potential benefits for knee osteoarthritis (OA). This study was undertaken to identify the major effective sub-fraction of SSG. The 95% ethanol extract of SSG was successively fractionated into petroleum ether, ethyl acetate, n-butanol and aqueous fractions. Then we examined the effect of fractions on the inflammatory response and cell proliferation of primary human osteoarthritic chondrocytes stimulated by interleukin (IL)-1β, a recognized and common inducer of OA in vitro. Results showed that the petroleum ether, ethyl acetate and n-butanol extracts of SSG promoted cell-proliferation measured by MTT [3-(4,5- dimethylthiazolyl-2)-2,5-diphe nyltetrazoliumbromide] assay, increased the transcript levels and nuclear translocation of cyclin D1, and inhibited the production of pro inflammatory mediator prostaglandin (PG)E2 and nitric oxide (NO) induced by IL-1β in human OA chondrocytes, suggesting SSG extracts possessed chondro protective and anti-inflammatory properties. The ethyl acetate fraction was superior to other fractions. These data indicated SSG extracts might become a potential treatment option for humans with OA.
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Fan, W., Qian, Y., Wang, J., Yang, X., Gui, T., & He, B. (2016). Chondroprotective and Anti-Inflammatory Activities of Extracts from Semen Sojae Germinatum on IL-1 β- Stimulated Human Osteoarthritis Chondrocytes. Indian Journal of Pharmaceutical Education and Research, 50(3), 397-402. https://doi.org/10.5530/ijper.50.3.12
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