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Application of TLC-Densitometry for Analysis of Estradiol Hemihydrate in Dosage Forms

1, 1,2, 1,2, 3, 4

  1. 1Medical University-Sofia, Faculty of Pharmacy, Department of Pharmaceutical Chemistry.
  2. 2Medical University-Plovdiv, Faculty of Pharmacy, Department of Pharmacognosy and Pharmaceutical Chemistry, 15A Vasil Aprilov Str., Plovdiv 4002, BULGARIA.
  3. 3Medical University-Plovdiv, Medical College, 15A Vasil Aprilov Str., Plovdiv 4002, BULGARIA.
  4. 4Clinic of Internal Diseases UMHATEM ”N. I. Pirogov”-SOFIA, BULGARIA.

CORRESPONDENCE

Dobrina Tsvetkova,

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University-Sofia, Dunav str. N : 2, 1000, Sofia, BULGARIA.

dobrinka30@abv.bg

Received: 23-04-2016; Accepted: 23-06-2016.

Volume 50, Issue 3 · pp. 482-488 · 2016 · DOI: 10.5530/ijper.50.3.23

ABSTRACT

The aim of current study was the application of validated TLC-densitometric method for identification and determination of Estradiol hemihydrate in dosage forms. The applied TLC conditions were: Silicagel G60F254 glass plates; mobile phase: chloroform : acetone = 90 : 10 v/v, migration distance of mobile phase: 120 mm, UV-detection at  = 254 nm. All of the experimental results for the content of Estradiol hemihydrate correspond to the respective confidence interval: Estrofem table: 1.78 mg ÷ 2.12 mg; Femoston F1 table: 1.88 mg ÷ 2.2 mg; Femoston F2 table: 1.99 mg ÷ 2.19 mg; Trisequens T1 table: 1.78 mg ÷ 2.18 mg; Trisequens T2 table: 1.92 mg ÷ 2.12 mg; Trisequens T3 table: 0.97 mg ÷ 1.17 mg. The proposed validated TLC-densitometric method is appropriate for quality control of Estradiol hemihydrate in commercially available tablets.

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    Tsvetkova, D. D., Obreshkova, D. P., Ivanova, S. A., Hadjieva, B., & Atanasov, P. Y. (2016). Application of TLC-Densitometry for Analysis of Estradiol Hemihydrate in Dosage Forms. Indian Journal of Pharmaceutical Education and Research, 50(3), 482-488. https://doi.org/10.5530/ijper.50.3.23

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    © 2016 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0