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Response Surface Methodology Optimization of Thebaine Biotransformation into Codeine and Morphine Using Bacillus sp. FAR

1, 2, 3, 4

  1. 1Department of Microbiology, Faculty of Biological Science, Alzahra University, Vanak, Tehran, IRAN.
  2. 2Department of Biotechnology, Faculty of Biological Science, Alzahra University, Vanak, Tehran, IRAN.
  3. 3Birjand Infectious Diseases Research Center, Birjand University of Medical Sciences, Ghafary Ave., Birjand, IRAN.
  4. 4Departmrnt of Nanobiotechnology, Protein Research Center (PRC), Shahid Beheshti University, G.C., Tehran, IRAN.

CORRESPONDENCE

Jamshid Fooladi

Department of Biotechnology, Faculty of Biological Science, Alzahra University, Vanak, Tehran, IRAN.

ataee1357@yahoo.com

Received: 29-01-2017; Accepted: 30-03-2017.

Volume 51, Issue 4 · pp. 684-691 · 2017 · DOI: 10.5530/ijper.51.4.101

ABSTRACT

Background: Codeine and morphine are important pharmaceuticals having analgesic characteristics.Aim: The current study used response surface methodology to optimize the biotransformation of thebaine into codeine and morphine using Bacillus sp. FAR.Methods: A central composite design was used to determine the optimal concentrations of buffer and thebaine, pH, time, temperature, and biomass. Improvement in transformation was monitored using HPLC-DAD.Results: The optimized conditions employed were pH 7.7 at 36°C for 30 h using a thebaine concentration of 80 μg/mL, 45 mM tris of buffer and 2.9% w/v biomass. The optimal conditions for codeine and morphine production were 2.995 and 1.113 μg, respectively.Conclusion: Optimization demonstrated that pH and biomass had the strongest effects on biotransformation.

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    Ataee, N., Fooladi, J., Namaei, M. H., & Mirzajani, F. (2017). Response Surface Methodology Optimization of Thebaine Biotransformation into Codeine and Morphine Using Bacillus sp. FAR. Indian Journal of Pharmaceutical Education and Research, 51(4), 684-691. https://doi.org/10.5530/ijper.51.4.101

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    © 2017 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0