Simultaneous Determination of Some 5-Nitroimidazole Derivatives and Ciprofloxacin by High Performance Liquid Chromatography
1, 1, 2, 2
- 1Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav st., 1000 Sofia, BULGARIA.
- 2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav st., 1000 Sofia, BULGARIA.
CORRESPONDENCE
Stanislav Bozhanov
Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav st., 1000 Sofia, BULGARIA.
Received: 05-12-2017; Accepted: 17-05-2018.
Volume 52, Issue 4s1 (Supplement) · pp. s23-s28 · 2018 · DOI: 10.5530/ijper.52.4s.72
ABSTRACT
Objective: An isocratic high performance liquid chromatographic method for simultaneous determination of Metronidazole, Tinidazole and Ciprofloxacin was developed.Method: Separation was achieved with a C18 (250x4.6 mm, 5 μm) column. Optimal chromatographic conditions were: mobile phase consisting of acetonitrile: 0.3% o-phosphoric acid modified with 0.1% triethylamine (20:80 v/v) at a flow rate of 0.7 mL min-1, at 30°C and wavelength of 300 nm.Results: The retention times were 4.92 min for Metronidazole, 8.78 min for Tinidazole and 9.76 min for Ciprofloxacin. The responses were linear (R2 = 0.9999) in the range of 12.5 – 100 μg mL-1. The limits of detection were 0.012 µg mL-1 for Metronidazole and Tinidazole and 0.04 µg mL-1 for Ciprofloxacin. The limits of quantification were 0.125 µg mL-1 for Metronidazole and Tinidazole and 0.4 µg mL-1 for Ciprofloxacin. The recovery (%) was within the range of 99.57 – 100.3% for all analytes.Conclusion: The method developed can successfully be used for routine analysis and quality control of Metronidazole, Tinidazole and Ciprofloxacin.
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Bozhanov, S., Maslarska, V., Tsvetkova, B., & Peikova, L. (2018). Simultaneous Determination of Some 5-Nitroimidazole Derivatives and Ciprofloxacin by High Performance Liquid Chromatography. Indian Journal of Pharmaceutical Education and Research, 52(4s1), s23-s28. https://doi.org/10.5530/ijper.52.4s.72
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