The Effectiveness of Chitosan- Pinus merkusii Extract Nanoparticle as Antioxidant and Anti-caspase 3 in against Lead Acetate Toxicity on the Lung of Wistar Rat
1, 2, 3, 4, 5
- 1Department of Pharmacology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, INDONESIA.
- 2Department of Pharmacy Biology, Faculty of Pharmacy, Hang Tuah University, Surabaya, INDONESIA.
- 3Department of Conservative Dentistry, Faculty of Dentistry, Airlangga University, Surabaya, INDONESIA.
- 4Study Program of Environmental Health, Polytechnic of Health, Surabaya, INDONESIA.
- 5Department of P3M, STIKES Delima Persada, Gresik, INDONESIA.
CORRESPONDENCE
Sri Agus Sudjarwo
Department of Pharmacology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, INDONESIA.
Received: 20-10-2018; Accepted: 20-03-2019.
Volume 53, Issue 2s (Supplement) · pp. s135-s142 · 2019 · DOI: 10.5530/ijper.53.2s.58
ABSTRACT
Background: The antioxidants can be used for protective in oxidative stress that is one of the important mechanisms of lead acetate-induced lung toxicity.Objective: The current study was carried out to evaluate the effectiveness of Chitosan- Pinus merkusii extract nanoparticle as antioxidant and anti-caspase 3 in against lead acetate-induced lung toxicity on wistar rats.Methods: Chitosan- Pinus merkusii nanoparticle were characterized by Dynamic Light Scattering (DLS) and Scanning Electron Microscope (SEM). The male rats were divided into control group (Rats were given with distilled water); lead acetate group (Rats were injected with lead acetate 20 mg/kg BW i.p) and the treatment group (Rats were given the Chitosan- Pinus merkusii nanoparticle 150 mg; 300 mg; 600 mg/kg BW orally and were injected with lead acetate 20 mg/kg BW). The lung tissues were collected to evaluate the malondialdehyde (MDA), Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx), histological evaluations of lung damage and immunohistochemical of the expression of caspase 3.Results: The results of DLS showed that the size of Chitosan- Pinus merkusii nanoparticle was 430.5±24.8 nm. SEM images of the Chitosan- Pinus merkusii nanoparticles showed an irregular shape and the morphology surface showed the rough surface. Administration of lead acetate resulted in a significant increase in MDA level, caspase 3 expression and decrease in level of SOD and GPx. Treatment with the Chitosan- Pinus merkusii nanoparticle 600 mg/kg BW but not 150 and 300 mg/kg BW significantly decreased the MDA levels, caspase 3 expression and also increase in level of SOD and GPx were compared with lead acetate group. The lead acetate-induced thickened the alveolar septa and loss of the normal structure of lung cells (Necrosis), whereas treated with Chitosan-Pinus merkusii nanoparticle inhibited lung cell necrosis.Conclusion: From the results of this study demonstrated that Chitosan- Pinus merkusii nanoparticle could be a potent against lead acetate- induced lung toxicity, through increasing antioxidant and inhibiting caspase 3 expression.
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Sudjarwo, S. A., Wardani, G., Eraiko, K., Koerniasari, K., & Ernawati, E. (2019). The Effectiveness of Chitosan- Pinus merkusii Extract Nanoparticle as Antioxidant and Anti-caspase 3 in against Lead Acetate Toxicity on the Lung of Wistar Rat. Indian Journal of Pharmaceutical Education and Research, 53(2s), s135-s142. https://doi.org/10.5530/ijper.53.2s.58
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