In vitro Metabolism of Risperidone on Isolated Microsomes of Rat Liver: Metabolite Identification and Profiling by RP-HPLC and LC-MS Techniques
1, 2, 3, 1
- 1Department of Pharmaceutical Chemistry, S.M.B.T. College of Pharmacy, Nashik, Maharashtra, INDIA.
- 2Department of Pharmaceutical Chemistry, Sandip Foundation’s, Sandip Institute of Pharmaceutical Sciences, Nashik, Maharashtra.
- 3Department of Pharmaceutics, Sandip Foundation’s, Sandip Institute of Pharmaceutical Sciences, Nashik, Maharashtra, INDIA.
CORRESPONDENCE
Prutha Shirodkar
Department of Pharmaceutical Chemistry, S.M.B.T. College of Pharmacy, Nashik, Maharashtra, INDIA.
Received: 07-07-2018; Accepted: 22-01-2019.
Volume 53, Issue 2s (Supplement) · pp. s179-s185 · 2019 · DOI: 10.5530/ijper.53.2s.64
ABSTRACT
Objective: The objective of present research work is to study in vitro metabolism of risperidone on isolated microsomes of rat liver.Methods: Its biotransformation was examined using isolated microsomes derived from rat livers. Their separation was accomplished on Jasco RP-HPLC C-18 column (250mm x 4.6mm x 5µm) using methanol: 50mM Potassium dihydrogen phosphate (50:50 %v/v) mobile phase, detected at a wavelength of 280nm.Results: In vitro metabolism of RIS was investigated using the cytochrome P450 (CYP450) enzymes supplemented with an NADPH- generating system. Incubation of RIS with rat liver microsomes resulted in the formation of 2 major hydroxy metabolites.Conclusion: Formation of all the metabolites is correlated with Phase I biotransformation CYP enzyme subfamilies. The method was extended to LC-MS for further characterization of the metabolites using standards and the probable metabolites were proposed.
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Shirodkar, P., Chavan, H., Mogal, R., & Dhake, A. (2019). In vitro Metabolism of Risperidone on Isolated Microsomes of Rat Liver: Metabolite Identification and Profiling by RP-HPLC and LC-MS Techniques. Indian Journal of Pharmaceutical Education and Research, 53(2s), s179-s185. https://doi.org/10.5530/ijper.53.2s.64
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