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HPLC Method for Determination of the Chemical Stability of Antimicrobial Peptide α-Defensin 2

1, 2, 2, 1

  1. 1Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav St., 1000 Sofia, BULGARIA.
  2. 2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav St., 1000 Sofia, BULGARIA.

CORRESPONDENCE

Vania Maslarska

Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav St., 1000 Sofia, BULGARIA.

vmaslarska@mail.bg

Received: 23-08-2019; Accepted: 08-01-2019.

Volume 54, Issue 2 · pp. 465-470 · 2020 · DOI: 10.5530/ijper.54.2.53

ABSTRACT

Aim: The aim of this research study was to develop a simple, rapid, precise, accurate and economical RP-HPLC method, with a simple mobile phase for the identification and determination of the antimicrobial peptide α-defensin-2.Materials and Methods: Separation was carried out at 25°C, using column Luna 5U (C18, 250x4.6, 5 μm) with mobile phase consisting of acetonitrile: water (40: 60 v/v). The detector was set at 210 nm. The flow rate was 1.2 ml/min and injection volume was 20 μl.Results: The developed method was validated as per ICH guidelines and the kinetic behavior of α-defensin 2 was studied in respect of different pH.Conclusion: The obtained data can serve for creating an in vivo monitoring program and quality control for different studies and preparations.

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    Maslarska, V., Amerikova, M., Pencheva, I., & Bozhanov, S. (2020). HPLC Method for Determination of the Chemical Stability of Antimicrobial Peptide α-Defensin 2. Indian Journal of Pharmaceutical Education and Research, 54(2), 465-470. https://doi.org/10.5530/ijper.54.2.53

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    © 2020 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0