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Novel Validated UV Spectroscopic Method for Routine Analysis of Decitabine Drug Substance

1, 1, 1

  1. 1Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, INDIA.

CORRESPONDENCE

Muddukrishna Badamane Sathyanarayana

Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, INDIA.

krishna.mbs@manipal.edu

Received: 08-04-2020; Accepted: 07-09-2020.

Volume 54, Issue 3s (Supplement) · pp. s667-s670 · 2020 · DOI: 10.5530/ijper.54.3s.166

ABSTRACT

Objectives: In the proposed study, a basic, novel, protected economic, delicate and cost-effective UV-Spectrophotometric technique for the analysis of Decitabine which is antineoplastic drug.Methods: The created method was approved according to ICH rules. The decitabine indicated maximum absorption at 221 nm. This strategy can successfully apply for estimation of Decitabine in active pharmaceutical ingredient (API) form for routine examination with UV identification at 221 nm. A Shimadzu UV-Visible spectrophotometer 1800 with 1 cm quartz cells and Acetonitrile (ACN) as diluent was utilized in this technique.Results: The Developed technique obeyed Beer's-Lambert's law in the conc. of 5.0-15.0 ug/ml, having R2 of 0.9993 and 99.82%±0.75 recovery of decitabine was found. Different approval parameters like linearity, accuracy, precision and robustness were performed and were found to be within the limits as per the guidelines of ICH.

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    Awasthi, A., HM, D., & Sathyanarayana, M. B. (2020). Novel Validated UV Spectroscopic Method for Routine Analysis of Decitabine Drug Substance. Indian Journal of Pharmaceutical Education and Research, 54(3s), s667-s670. https://doi.org/10.5530/ijper.54.3s.166

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    © 2020 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0