Development and Validation of UV Spectrophotometric Method for Estimation of Tea Tree Oil in Bulk and Cosmeceutical Creams
1, 1, 1, 1
- 1Department of Pharmaceutics, Government College of Pharmacy, Rajiv Gandhi University of Health Sciences, Bengaluru, Karnataka, INDIA.
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Basappa Palur Manjula
Department of Pharmaceutics, Government College of Pharmacy, Rajiv Gandhi University of Health Sciences, Bengaluru, Karnataka, INDIA.
Received: 23-06-2020; Accepted: 03-01-2021.
Volume 55, Issue 1s (Supplement) · pp. S285-S292 · 2021 · DOI: 10.5530/ijper.55.1s.61
ABSTRACT
Background: Tea tree oil, also known as Melaleuca alternifolia essential oil is widely used in skin care cosmeceuticals for its antibacterial, antifungal, analgesic and anti-inflammatory effect. The complex and variable composition of the oil poses many challenges to the analytical chemist and a recent survey of analytical methods indicated only a few simple and validated methods for estimation of the oil.Aim: A quality by design approach has therefore been adopted to develop a simple and novel UV spectrophotometric method for estimation of tea tree oil in bulk and cosmeceutical creams.Materials and Methods: UV spectrophotometric method was developed for estimation of Tea tree oil using dichloromethane- methanol (54 % v/v) solvent system. The method was then validated under optimized conditions for accuracy, precision and ruggedness, limit of detection and limit of quantification as per ICH: Q2 (R1) guidelines.Results: A characteristic spectral peak was observed at 267 nm and linearity was established over a concentration range of 10-160 mcg/ml with R2 value of 0.9961. Accuracy based on recovery studies, 100.5-113.6 %, precision and ruggedness based on % RSD values less than 2 %, LOD, 0.1277mcg/ml and LOQ and 4.195 mcg/ml indicated that the method is sensitive enough to be used for routine estimation.Conclusion: The method is robust and has been used to determine the essential oil content of two cosmeceutical creams.
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Manjula, B. P., Joshi, V. G., Setty, R. S. S., & Geetha, M. (2021). Development and Validation of UV Spectrophotometric Method for Estimation of Tea Tree Oil in Bulk and Cosmeceutical Creams. Indian Journal of Pharmaceutical Education and Research, 55(1s), S285-S292. https://doi.org/10.5530/ijper.55.1s.61
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