Development and Validation of Ultraviolet Spectroscopic Method for Estimation of Methoxsalen in Bulk Using Methanol and Phosphate Buffer (pH 7.4)
1, 1
- 1Department of Pharmaceutics, Prin. KM Kundnani College of Pharmacy (Affiliated to University of Mumbai), Mumbai, Maharashtra, INDIA.
CORRESPONDENCE
Dr. Nalini S Kurup
Department of Pharmaceutics, Prin. KM. Kundnani College of Pharmacy, Colaba, Mumbai-400 005, Maharashtra, INDIA.
Received: 25-06-2020; Accepted: 13-05-2021.
Volume 55, Issue 2s (Supplement) · pp. s572-s579 · 2021 · DOI: 10.5530/ijper.55.2s.129
ABSTRACT
Background: Psoriasis is an inflammatory condition of a body wherein erythematous, sharply demarcated papules and round-shaped plaques appear onto skin due to the hyperproliferation of epidermis. Psoralens are the class of furanocoumarins that are effectively used in the treatment of psoriasis and vitiligo in conjunction with exposure to a dose of ultraviolet radiation. It is crucial to develop and validate the suitable analytical method for its quantitative estimation. The present work aims to develop and validate simple, rapid, accurate and cost-effective (as compared to other sophisticated techniques of analysis) Ultraviolet-Visible Spectrophotometry in methanol and phosphate buffer saline (pH 7.4).Materials and Methods: Methanol and phosphate buffer saline (pH 7.4) were the two solvents chosen for method development and results of validation parameters were calculated.Results: Methoxsalen showed maximum absorbance at 249 nm in methanol and at 247 nm in phosphate buffer saline (pH 7.4). The percent recoveries for determination of accuracy and repeatability were found within a suitable confidence interval of 98 %- 102 %.Conclusion: The developed method was evaluated quantitatively for accuracy, precision, robustness, ruggedness and other parameters.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Unde, S., & Kurup, N. (2021). Development and Validation of Ultraviolet Spectroscopic Method for Estimation of Methoxsalen in Bulk Using Methanol and Phosphate Buffer (pH 7.4). Indian Journal of Pharmaceutical Education and Research, 55(2s), s572-s579. https://doi.org/10.5530/ijper.55.2s.129
Copyright and licence
© 2021 Author(s)
This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0