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Indian Journal of Pharmaceutical Education and ResearchAn Official Publication of Association of Pharmaceutical Teachers of India (APTI)
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Binary Solvent-assisted Graphene Synthesis by Direct Exfoliation of Graphite Powder and its Cytotoxicity on SiHa Cell Line

1, 1, 1

  1. 1Department of Chemistry, Netaji Subhas University of Technology, New Delhi, INDIA.

CORRESPONDENCE

Anjana Sarkar

Department of Chemistry, Netaji Subhas University of Technology, New Delhi, INDIA.

prabhat.yadav.trf18@nsut.ac.in

Received: 10-03-2022; Accepted: 05-06-2022.

Volume 56, Issue 3 · pp. 732-738 · 2022 · DOI: 10.5530/ijper.56.3.122

ABSTRACT

A cost-effective mass production of pristine graphene nanosheet is still an important challenge to harness its maximum potential in medical sciences. Here, we have used a binary solvent mixture of low dielectric constant (1,2,4- trichlorobenzene,ε = 2.24 and benzylamine,ε = 4.6) to produce a high quantity (0.6 mg/ml) of graphene under ultrasonication without compromising its quality. Obtained sample were characterized through XRD, UV-Vis, Raman, FT-IR, TEM-SAED, and cytopotency was evaluated on SiHa cell line. IG/I2D < 1.0 (~ 0.39) was achieved on more than 250 μm2 area, indicating the uniformity and obtained ID/IG ratio was 1.21. No toxicity was found in the case of 24 hr on the SiHa cell line. EC50 computation was 84.90 μg /ml for 48 hr and 62.03 μg /ml for 72 hr. Exfoliated graphene remained stable in binary solvent even after 1 year. The proposed experiment and obtained data can be powerful tools to explore the synergic effect of binary solvent exfoliation in combination to establish graphene as a biomaterial for neuronal network, tissue engineering, drug delivery, and regenerative medicines, to name a few.

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    Kumar, P., Jain, P., & Sarkar, A. (2022). Binary Solvent-assisted Graphene Synthesis by Direct Exfoliation of Graphite Powder and its Cytotoxicity on SiHa Cell Line. Indian Journal of Pharmaceutical Education and Research, 56(3), 732-738. https://doi.org/10.5530/ijper.56.3.122

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    © 2022 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0