Development and Validation of UV-spectrophotometric Method for the Estimation of Wintergreen Oil in Pharmaceutical Formulation
1, 1, 2, 1
- 1Department of Pharmaceutics, Government College of Pharmacy, Bengaluru, Karnataka, INDIA.
- 2Department of Pharmacology, Government College of Pharmacy, Bengaluru, Karnataka, INDIA.
CORRESPONDENCE
* Dr. Vijaya G. Joshi
Department of Pharmaceutics, Government College of Pharmacy, Bengaluru-560027, Karnataka, INDIA.
Received: 27-07-2022; Accepted: 24-01-2023.
Volume 57, Issue 2 · pp. 591-597 · 2023 · DOI: 10.5530/ijper.57.2.72
ABSTRACT
Objectives: The study aimed to develop and validate a simple UV-spectrophotometric method for quantifying Wintergreen oil in micro/nanosponges.Materials and Methods: The method was developed using acetonitrile as a solvent system and validated for various parameters such as linearity, precision, repeatability, the Limit of Detection (LOD), and the Limit of Quantification (LOQ), and accuracy according to ICH guidelines.Results: The oil showed an absorption maximum at 237nm. Linearity between concentration and absorbance was established within a concentration range of 2 to 28 mcg/mL and showed a regression coefficient of 0.9746. The recovery of 97.14-107.82% and % RSD of less than 2% for repeatability, intraday, inter-day, and ruggedness revealed that the method is accurate and precise. The LOD and LOQ were determined as 0.103 mcg/mL and 0.312 mcg/mL respectively. Insignificant changes in absorption values with deliberate variation in absorption maximum indicate the method's robustness. The entrapment efficiency and loading capacity ranged between 88-97.82% and 3.085-24.35%.Conclusion: The validation results conclude that the method is simple, sensitive, precise, accurate, and robust. Hence, the method was used for quantifying wintergreen oil in micro/nanosponges. The procedure can further be adapted for analysing wintergreen oil in other pharmaceutical preparations and commercial products.
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Deepali, Joshi, V. G., Setty, S. R., & Geetha, M. (2023). Development and Validation of UV-spectrophotometric Method for the Estimation of Wintergreen Oil in Pharmaceutical Formulation. Indian Journal of Pharmaceutical Education and Research, 57(2), 591-597. https://doi.org/10.5530/ijper.57.2.72
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