Method Development and Validation for the Quantification of Abametapir in Biological Matrices by LC-ESI-MS/MS
1, 2,3, 3
- 1Department of Chemistry, Bir Tikandrajit University, Canchipur, Imphal West, Manipur, INDIA.
- 2Department of Pharmacy, Bir Tikandrajit University, Canchipur, Imphal West, Manipur, INDIA.
- 3Department of Pharmaceutical Analysis, TRR College of Pharmacy, Meerpet, Hyderabad, Telangana, INDIA.
CORRESPONDENCE
Dr. Darna Bhikshapathi
Department of Pharmacy, Bir Tikandrajit University, Canchipur, Imphal West, Manipur, INDIA.,
Received: 02-03-2023; Accepted: 12-05-2024.
Volume 58, Issue 3s (Supplement) · pp. s1028-s1033 · 2024 · DOI: 10.5530/ijper.58.3s.102
ABSTRACT
Aim: A precise, linear and specific liquid chromatography-tandem mass spectrometry procedure was executed and subjected for validation for the quantification of plasma.Materials and Methods: The chromatography elution was carried out using a C18-Discovery column of 15 cm in length and 2.1 mm in internal diameter, packed with stationary phase particles of 5 μm size, at a flow rate of 0.80 mL/min. An isocratic elution process was conducted with a mobile phase solution consisting of methanol and 0.10% V/V HCOOH in a ratio of 90:10. The separation of Abametapir and Metformin was achieved by a liquid-liquid extraction process using ethyl acetate as the solvent.Results: A triple quadrupole mass spectrometer was used for the measurement of ions. Electrospray ionization is a technology that ionizes positively, and it was used in Multiple Reaction Monitoring (MRM) with parent/product ionic transitions m/z 185.1→106.06 for Abametapir and 130.1→60.20 for the Metformin internal standard. A calibration graph was constructed with values ranging from 2.05 to 82.00 ng/mL, resulting in the equation y=0.0149x+0.00221, with a r2 value above 0.99. The recovery values of Abametapir exceeded 94.27%, with its accuracy assessed in terms of relative error ranging from -4.04% to 6.52%.Conclusion: The accuracy, recovery and sensitivity values of Abametapir in the plasma sample, as shown by the established approach, highlight its significance in pharmacokinetic and bioequivalence research.
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Krishnan, V. S., Bhikshapathi, D., & Cheruku, S. (2024). Method Development and Validation for the Quantification of Abametapir in Biological Matrices by LC-ESI-MS/MS. Indian Journal of Pharmaceutical Education and Research, 58(3s), s1028-s1033. https://doi.org/10.5530/ijper.58.3s.102
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