Hepatoprotective Properties of Acacia nilotica Bark Extract against H2O2 Induced Oxidative Damage in Normal Liver (LO2) and Cancer (HepG2) Cells
1, 1, 2, 3
- 1Department of Oncology, Qingdao Eighth People’s Hospital, Qing Dao, CHINA.
- 2Department of Pharmacy, Qingdao Eighth People’s Hospital, Qing Dao, CHINA.
- 3Department of Oncology, Qingdao Municipal Hospital, Qing Dao, CHINA.
CORRESPONDENCE
Mrs. Yinghui Bi
Department of Oncology, Qingdao Municipal Hospital, Qing Dao, 266071, CHINA.
Received: 25-03-2024; Revised: 12-07-2024; Accepted: 08-10-2024.
Volume 59, Issue 1 · pp. 370-376 · 2025 · DOI: 10.5530/ijper.20256767
ABSTRACT
Background: Liver cancer's severity has prompted interest in herbal remedies for hepatotoxicity and the disease. Acacia nilotica (Vachellia nilotica), valued for its traditional medicinal properties, particularly in its aerial components, is being investigated for its hepatoprotective and anti-cancer effects. However, research on A. nilotica bark extracts and their mechanisms of action is limited.Materials and Methods: The study aimed to determine the Total Phenolic Content (TPC), Total Flavonoid Content (TFC), and antioxidant properties of A. nilotica bark extract using biochemical assays. Additionally, it examined the protective effects of A. nilotica bark extract on normal Liver Cells (LO2) and its toxicity against liver cancer cells (HepG2) using cell culture techniques.Results: The extract showed significant antioxidant properties (EC50-16.34), with a total phenolic content of 159.98±9.91 mg GAE/DW and a flavonoid content of 16.93 mg QE/g dry weight. It exhibited moderate toxicity towards HepG2 cells at concentrations >100 µg/mL but was non-toxic to LO2 cells. Moreover, it prevented oxidative damage in LO2 cells induced by H2 O2 .Conclusion: These findings highlight the therapeutic potential of A. nilotica bark extract in liver cancer and hepatotoxicity treatment, warranting further clinical investigation.
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Cai, H. J., Yan, Q., Du, Z., & Bi, Y. (2025). Hepatoprotective Properties of Acacia nilotica Bark Extract against H2O2 Induced Oxidative Damage in Normal Liver (LO2) and Cancer (HepG2) Cells. Indian Journal of Pharmaceutical Education and Research, 59(1), 370-376. https://doi.org/10.5530/ijper.20256767
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