Development and Validation of a High-Performance Liquid Chromatographic Method for Content Assay of Ceftriaxone in Pharmaceutical Dosage
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- 1Department of Chemistry, S. S. Margol College of Arts, Science and Commerce, Shahabad, Kalaburagi, Karnataka, INDIA.
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drbhiremath25@gmail.com
Department of Chemistry, S. S. Margol College of Arts, Science and Commerce, Shahabad, Kalaburagi, Karnataka, INDIA.
Received: 30-10-2023; Revised: 23-01-2024; Accepted: 19-10-2024.
Volume 59, Issue 1 · pp. 94-100 · 2025 · DOI: 10.5530/ijper.20255575
ABSTRACT
Aim: A sensitive and accurate RP-HPLC method was developed and validated for the quantitative measurement of Ceftriaxone sodium (CEF) in pharmaceutical dosage forms and bulk medicine.Materials and Methods: Ceftriaxone sodium was separated on a 245 nm photodiode array detector using a Waters XTerra RP-18 (5 µm 250x4.6 mm internal diameter) column.Results: The method generated an excellent linear response in the concentration range of 0.2-20 µg/mL with remarkable precision of 0.16-0.7% and accuracy in the percent recovery range of 99.88-99.97%.Conclusion: We find the reverse phase HPLC technique to be highly sensitive, accurate, precise and user-friendly and hence recommend it. Therefore, it could be useful in a quality control lab.
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Hiremath, B. (2025). Development and Validation of a High-Performance Liquid Chromatographic Method for Content Assay of Ceftriaxone in Pharmaceutical Dosage. Indian Journal of Pharmaceutical Education and Research, 59(1), 94-100. https://doi.org/10.5530/ijper.20255575
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