Exploring the Green Potential of Quercetin-Enhanced Strontium Oxide Nanoparticle Scaffold on Mesenchymal Stem Cells BMP-2 Expression
1, 1
- 1Department of Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences [SIMATS], Saveetha University, Chennai, Tamil Nadu, INDIA.
CORRESPONDENCE
Dr. Priyangha P.T
Department of Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences [SIMATS], Saveetha University, Chennai, Tamil Nadu, INDIA.
Received: 06-06-2024; Revised: 19-02-2025; Accepted: 22-02-2025.
Volume 59, Issue 3s (Supplement) · pp. s989-s994 · 2025 · DOI: 10.5530/ijper.20257040
ABSTRACT
Aim: The study aimed to create a scaffold infused with Quercetin-enhanced strontium oxide nanoparticles and assess its impact on BMP-2 expression in MSCs.Materials and Methods: Scaffold fabrication involved mixing 1% Hyaluronic acid, 0.5% carrageenan and 2% gelatin in a ratio of 6:1:3, adding 10 mg of quercetin-doped SrO nanoparticles and freezing at -20ºC for 12 hr and -80ºC for 24 hr. Dental Pulp Stem Cells (DPSC) cultured in DMEM F12 supplemented with 10% FBS were seeded onto the scaffolds treated with UV radiation. Osteogenic marker gene expression was analyzed using qPCR at 1, 3 and 5 days post-seeding, comparing control and test groups. The comparative Ct method was used to quantify gene expression using normalization of GAPDH. Experiments were conducted in triplicate and repeated thrice for reliability.Results: On Day 1, the control group showed a BMP-2 concentration of 5.8 ng/μL, decreasing slightly to 5.1 ng/μL by Day 3 and increasing to 9.6 ng/μL by Day 5. In contrast, the experimental group displayed a higher BMP-2 concentration of 7.4 ng/μL on Day 1, dropping to 0.3 ng/μL by Day 3, but significantly increasing to 27.3 ng/μL by Day 5.Conclusion: Combining green-synthesized strontium oxide nanoparticles with Quercetin enhances bone regeneration and differentiation, creating a microenvironment conducive to tissue repair. Quercetin's anti-inflammatory and antioxidant properties align with greener biomedical trends, promising to address bone defects and improve patient outcomes
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Vasudevan, R., & Thangavel, P. P. (2025). Exploring the Green Potential of Quercetin-Enhanced Strontium Oxide Nanoparticle Scaffold on Mesenchymal Stem Cells BMP-2 Expression. Indian Journal of Pharmaceutical Education and Research, 59(3s), s989-s994. https://doi.org/10.5530/ijper.20257040
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