A Validated HPTLC Method for Simultaneous Estimation of Berberine and Trigonelline in Polyherbal Mixture A Tool for Quality Control and Standardization
1, 1, 2
- 1Department of Pharmacognosy, KLE College of Pharmacy Belagavi, KLE Academy of Higher Education and Research, Belagavi, Karnataka, INDIA.1Department of Pharmacognosy, KLE College of Pharmacy Belagavi, KLE Academy of Higher Education and Research, Belagavi, Karnataka, INDIA.
- 2Dr. Prabhakar Kore Basic Science Research Centre KLE Academy of Higher Education and Research, Belagavi, Karnataka, INDIA.
CORRESPONDENCE
Dr. Sunil Satyappa Jalalpure
Department of Pharmacognosy, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, Belagavi, Karnataka, INDIA.
Received: 27-12-2024; Revised: 07-02-2025; Accepted: 12-05-2025.
Volume 60, Issue 1 · pp. 431-441 · 2026 · DOI: 10.5530/ijper.20260726
ABSTRACT
Introduction: Berberis aristata and Trigonella foenumgrecum have been used in traditional medicine for various purposes due to their diverse therapeutic benefits such as anti- inflammatory, antidiabetic, antihypertensive and digestive properties. Berberis aristata and Trigonella foenumgrecum consists of bioactive markers Berberine and Trigonelline respectively.Aim: This study aimed to develop a simple, rapid reliable and robust HPTLC method for the simultaneous quantification of biomarkers in polyherbal mixture.Materials and Methods: The separation of these active compounds from the polyherbal mixture was achieved using a mobile phase consisting of toluene: ethyl acetate: formic acid: methanol (6:3:0.3:1v/v/v/v) on pre-coated Silica G 60 F254 plates. Validation of the method encompassed assessments of linearity, accuracy, precision, limits of detection and quantification, robustness and specificity.Results: Berberine and Trigonelline displayed distinct Rf values of 0.88 and 0.29 respectively. The method demonstrated excellent linearity across the concentration range of 2-10 µg/band, with correlation coefficients of 0.9966 and 0.9973 for Berberine and Trigonelline respectively.Conclusion: Percentage recovery was found within the range of 98.84-102.92%. The proposed HPTLC method can be implemented for the concurrent estimation of Berberine and Trigonelline for routine quality control of herbal formulations containing these herbs.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Patil, S. B., Jalalpure, S. S., & Shetti, P. P. (2026). A Validated HPTLC Method for Simultaneous Estimation of Berberine and Trigonelline in Polyherbal Mixture A Tool for Quality Control and Standardization. Indian Journal of Pharmaceutical Education and Research, 60(1), 431-441. https://doi.org/10.5530/ijper.20260726
Copyright and licence
© 2026 Author(s)
This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0