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Inhibition of hesperidin on epithelial to mesenchymal transition of non-small cell lung cancer cells induced by TGF

1, 2, 3,4, 1

  1. 1The First Affiliated Hospital of Henan University of TCM, Zhengzhou 450000, China;.
  2. 2The Hospital of Henan Academy Institute of Traditional Chinese Medicine, Henan Zhengzhou 450000, China;.
  3. 3Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China;.
  4. 4Collaborative Innovation Center for Respiratory Disease Diagnosis And Treatment & Chinese Medicine Development of Henan Province, Zhengzhou 450046, China.

CORRESPONDENCE

Zhou-xin Ren, Henan

University of Traditional Chinese Medicine, Zhengzhou 450046, China;.

renzhouxin123@126.com

Received: 03-05-2016; Accepted: 09-09-2016.

Volume 50, Issue 4 · pp. 583-590 · 2016 · DOI: 10.5530/ijper.50.4.10

ABSTRACT

Transforming growth factor-β1 (TGF-β1) is a known potent inducer causing epithelial to mesenchymal transition(EMT) in some cancers. The present study was to evaluate the effect of hesperidin on EMT of non-small cell lung cancer (NSCLC) in vitro. In this study, A549 cells, a cellular line of non-small cell lung cancer, were used for evaluation of EMT induced by TGF-β1. MTT assay was used to evaluate the effect of hesperidin on cell viability with or without TGF-β1 co-culture. At the same time of TGF-β1 addition or after cellular morphological change by TGF-β1 stimulation, hesperidin was given to cells, respectively. Roundness of cells, E-cadherin, α-SMA, Col-I, MMP-9, TIMP-1 were evaluated EMT by TGF-β1 induction and effect of hesperidin on the transition. 40 and 80μM hesperidin showed significant inhibition on cellular viability without TGF-β1; co culture with TGF-β1, 40μM hesperidin showed significant inhibition on the viability but not showed significant inhibition after TGF-β1 stimulation. Whether co-culture with TGF-β1 or addition after TGF-β1, 40μM hesperidin showed inhibitory effect on epithelial to mesenchymal transition and inhibitory effect on extracellular matrix degradation of MMP-9. The above results suggest that hesperidin could be a potential candidate for inhibited migration of non-small cell lung cancer.

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    Yu, H., Li, L., Ren, Z., & Shen, J. (2016). Inhibition of hesperidin on epithelial to mesenchymal transition of non-small cell lung cancer cells induced by TGF. Indian Journal of Pharmaceutical Education and Research, 50(4), 583-590. https://doi.org/10.5530/ijper.50.4.10

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    © 2016 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0