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Novel RP-HPLC Method Development and Validation of Meloxicam Suppository

1, 1, 1, 1, 2

  1. 1Department of Quality Assurance, Gangamai College of Pharmacy, Nagaon, Dist-Dhule (Maharashtra), INDIA.
  2. 2Department of Pharmacognosy, Smt. Sharadchandrika Suresh Patil College of Pharmacy, Chopda, Maharashtra, INDIA.

CORRESPONDENCE

Sufiyan Ahmad

Department of Quality Assurance, Gangamai College of Pharmacy, Nagaon, Dist-Dhule (Maharashtra), INDIA.

sufimpharm@rediffmail.com

Received: 09-02-2017; Accepted: 13-07-2017.

Volume 51, Issue 4 · pp. 644-649 · 2017 · DOI: 10.5530/ijper.51.4.95

ABSTRACT

Objective: A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of Meloxicam drug (MLX) in pharmaceutical mixture.Methods: Effective chromatographic separation achieved using a phenomenex luna C18 (4.6 mm, 250 mm, 5 μm) column with isocratic elution by the mobile phase composed of 0.02 M Potassium dihydrogen orthophosphate, pH adjusted to 4 with orthophosphoric acid (filtered): acetonitrile (50:50) respectively. The flow rate is 1.0 ml/min on detecting wavelength 220 nm.Results: The proposed HPLC method was statistically validated with respect to linearity, ranges, precision, accuracy, selectivity, LOD, LOQ and robustness. The retention time (RT) of Meloxicam was found to be 6.0 min. respectively. All parameters were found to be within the acceptance limit. The calibration curve was linear in ranges of 3-6, 6-9, and 15-18 mg/ ml for Meloxicam. The R2 of Meloxicam was found to be 0.996 respectively.Conclusion: A novel simple, simple, sensitive, precise, rapid, accurate and economical and reliable RP-HPLC method was developed and validated for the Meloxicam suppository.

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    Ahmad, S., Deepika, S., Amol, P., Kapil, W., & Md.Usman, M. R. (2017). Novel RP-HPLC Method Development and Validation of Meloxicam Suppository. Indian Journal of Pharmaceutical Education and Research, 51(4), 644-649. https://doi.org/10.5530/ijper.51.4.95

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    © 2017 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC BY 4.0