Simultaneous Determination of Amitriptyline Hydrochloride and Propranolol Hydrochloride in Commercial Formulation by Multitudinal UV and Multivariate FT-IR Spectroscopic Methods
1, 1, 2, 1, 1, 3
- 1Department of Pharmaceutical Analysis, Sri Padmavathi School of Pharmacy, Mohan Gardens, Tiruchanoor, Andhra Pradesh, INDIA.
- 2Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh, INDIA.
- 3Department of Pharmacology, Sri Padmavathi School of Pharmacy, Mohan Gardens, Tiruchanoor, Andhra Pradesh, INDIA.
CORRESPONDENCE
Dr. Sreenivasa Charan Archakam
Department of Pharmaceutical Analysis, Sri Padmavathi School of Pharmacy, Mohan Gardens, Tiruchanoor-517503, Andhra Pradesh, INDIA.
Received: 03-04-2023; Accepted: 16-01-2024.
Volume 58, Issue 2s (Supplement) · pp. s552-s557 · 2024 · DOI: 10.5530/ijper.58.2s.57
ABSTRACT
Aim: In the current study, a multivariate FTIR method and two different UV spectrophotometric methods were employed for the simultaneous determination of Amitriptyline Hydrochloride (ATH) and Propranolol Hydrochloride (PPH) in their combined formulation.Materials and Methods: The multivariate FTIR method based on the use of Classical Least Squares (CLS) was developed and executed in the lab solutions software for the simultaneous determination of ATH and PPH. CLS model was performed in the wavenumber range of 2550.04-3600.12 cm-1 and 969.64-1757 cm-1. In addition, two UV spectroscopic methods, namely absorbance correction method and crammer’s matrix method were developed and validated in the concentration ranges of 2-10μg/mL and 5-55μg/mL for ATH and PPH respectively.Results: The statistical parameters obtained from the applied multivariate FTIR-CLS model revealed the model accuracy and the assay results of ATH and PPH were found to be 92.32 (%w/w) and 97.35 (%w/w) respectively. The developed UV spectroscopic methods were validated as per ICH guidelines and all the validation parameters were found to be within the acceptance criteria.Conclusion: The methods employed in the current study were found to simple, economical, accurate and do not require any prior separation.
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Archakam, S. C., Palur, K., Galla, R., Padiri, S. S., Tulasi, P. K., & Diviti, R. (2024). Simultaneous Determination of Amitriptyline Hydrochloride and Propranolol Hydrochloride in Commercial Formulation by Multitudinal UV and Multivariate FT-IR Spectroscopic Methods. Indian Journal of Pharmaceutical Education and Research, 58(2s), s552-s557. https://doi.org/10.5530/ijper.58.2s.57
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