Comparative Study of Quercetin Content in Marketed Seeds of Linum usitatissimum L. (Flax), Salvia hispanica L. (Chia), and Helianthus annuus L. (Sunflower) and their Microgreens Using HPTLC from West Bengal
1, 1, 1
- 1Department of Pharmaceutical Chemistry, Guru Nanak Institute of Pharmaceutical Science and Technology, Kolkata, West Bengal, INDIA
CORRESPONDENCE
Dr. Prerona Saha Associate Professor
Department of Pharmaceutical Chemistry, Guru Nanak Institute of Pharmaceutical Science and Technology, Kolkata, West Bengal, INDIA.
Received: 26-09-2023; Revised: 05-01-2024; Accepted: 07-10-2024.
Volume 59, Issue 1s (Supplement) · pp. s221-s228 · 2025 · DOI: 10.5530/ijper.20255309
ABSTRACT
Background: Microgreens, the early developmental stage of edible plants, have gained prominence for their dense nutrient composition. Yet the variations in quercetin content among different microgreen species remain insufficiently explored.Aim: The aim of this research was to use HPTLC to compare the quercetin content of commercial flax, chia, and sunflower seeds as well as the comparable microgreens.Materials and Methods: Toluene, ethyl acetate, and formic acid were used as the mobile phase at a ratio of 5:4:0.2 (%v/v/v) for the chromatographic analysis, which was conducted using aluminum-backed silica gel 60F254 plates. To ensure that the results were precise, accurate, and reproducible, rigorous technique validation processes were carried out.Results: Total Flavonoid content in the three seeds and microgreens studied, and the highest value was 38.92±0.4 and 76.36±0.4gm QE/100g. Well separated and compact spots (Rf ) of quercetin (0.41±0.03) were detected. The regression equation obtained was y=0.0002x + 0.0001, with a correlation coefficient (R2 ) of 0.9833. The linearity range (µg/ spots) was 20-100. The LOD/ LOQ (ng/spot) were 37.66/114.15. Salvia hispanica L Seeds and microgreens (0.84±0.01 and 0.88±0.005% W/W) contained maximum amount of quercetin compared to Linus usitatissimum L. and Helianthus annus L. in ethanolic extract.Conclusion: The study revealed that the Salvia hispanica L. microgreen possess the highest quercetin content among the studied seeds and microgreens, while microgreens of all three plants are promising sources of quercetin, showcasing a remarkable increase compared to their seeds. Highlighting their potential as dietary sources rich in quercetin.
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Adhikari, T., Ray, P. B., & Saha, P. (2025). Comparative Study of Quercetin Content in Marketed Seeds of Linum usitatissimum L. (Flax), Salvia hispanica L. (Chia), and Helianthus annuus L. (Sunflower) and their Microgreens Using HPTLC from West Bengal. Indian Journal of Pharmaceutical Education and Research, 59(1s), s221-s228. https://doi.org/10.5530/ijper.20255309
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