Synthesis and Evaluation of 2, 5-substituted Pyrazolone for Neuroprotective Potential in SH-SY5Y Human Neuroblastoma Cells
1, 1, 2
- 1Department of Pharmacy, Annamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu, INDIA.
- 2Department of Pharmaceutical Chemistry, Kamalakshi Pandurangan College of Pharmacy, Tiruvannamalai, Tamil Nadu, INDIA.
CORRESPONDENCE
Mr. R. Manikandan
Research Scholar, Department of Pharmacy, Faculty of Engineering & Technology, Annamalai University, Annamalai Nagar-608002, Chidambaram, Tamil Nadu, INDIA.
Received: 28-11-2023; Revised: 31-01-2024; Accepted: 01-02-2024.
Volume 59, Issue 1s (Supplement) · pp. s323-s332 · 2025 · DOI: 10.5530/ijper.20256349
ABSTRACT
Aim: To carry out Synthesis and Evaluation of novel 2, 5-substituted Pyrazolone for Neuroprotective Potential in SH-SY5Y Human Neuroblastoma Cells.Materials and Methods: Alzheimer's Disease (AD) has become a serious public health concern as a result of people living longer than ever before. It is critically necessary to discover a way to halt and postpone the illness. The present study aims to determine if new synthetic analogue pyrazole derivatives may protect against toxicity caused by Aβ25-35 and its underlying mechanisms in neuroblastoma cells like SH-SY5Y. PyRx 0.9 software was used for molecular docking studies. The cells of SH-SY5Y were preincubated for 30 min with varying doses of the generated compounds (C1-C10) to induce neurotoxicity. They were then grown in Aβ25-35 (25 mol/L) for 48 hr. Cell viability was determined by MTT assay.Results: Compounds C5 and C8 exhibited a better binding score -8.4k/ cal compared to other analogues. Synthesized compounds C5 and C8 inhibited Aβ25-35-induced apoptosis in SH-SY5Y cells and protected neural cells from damage.Conclusion: The MTT assay confirmed that compounds C5 and C8 significantly reduced Aβ25-35-induced toxicity among human neuroblastoma SH-SY5Y cells, demonstrating its neuroprotective properties.
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Manikandan, R., Devi, K., & Rajalingam, D. (2025). Synthesis and Evaluation of 2, 5-substituted Pyrazolone for Neuroprotective Potential in SH-SY5Y Human Neuroblastoma Cells. Indian Journal of Pharmaceutical Education and Research, 59(1s), s323-s332. https://doi.org/10.5530/ijper.20256349
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