Phytofabrication of Magnetite Nanoparticles from Piper betle (L.) var. Mysore and Evaluation of Anti-Breast Cancer Potentials
1, 1, 1
- 1Department of Studies in Biotechnology, University of Mysore, Mysore, Karnataka, INDIA.
CORRESPONDENCE
Nagaraja Geetha
Department of Studies in Biotechnology University of Mysore, Mysore, Karnataka, INDIA.
Received: 01-05-2025; Revised: 30-06-2025; Accepted: 18-08-2025.
Volume 60, Issue 2s (Supplement) · pp. s533-s540 · 2026 · DOI: 10.5530/ijper.20261603
ABSTRACT
Background: Breast cancer is a predominant disease around the world, with early diagnosis aiding in reducing the spread and recurrence. Plant-mediated nanoparticles have shown promising results in treatment. The current study aimed to synthesize Magnetite Nanoparticles (MNPs) utilizing Piper betle var. Mysuru extract and evaluate its anti-breast cancer potentials.Materials and Methods: The green synthesis included Fe3+ and Fe2+ in a 2:1 proportion, with crude ethyl acetate extract as a reducing, stabilizing, capping, and tailing agent. The MNPs were characterized utilizing UV-vis spectrometry, SEM, EDX, XRD, FTIR, and DLS for various properties like size, crystal structures, presence of functional groups, surface topologies, etc.Results: The diameter of the newly synthesized nanoparticle was 90 nm in diameter, with a face-centered crystal lattice with different phytochemicals like alcohol, phenols, alkanes, etc. The cytotoxicity assay showed IC50 values of Doxorubicin, plant extract, and MNPs to be 16.77±0.56 μg/mL, 26.07±3.14 μg/mL, and 12.35±2.47 μg/mL, respectively. The hemolytic assay showed 8.8% and 12.28% hemolytic activity with plant extract and MNPs, respectively.Conclusion: The MNPs' affordability, Eco-friendliness, and ease of synthesis make them ideal candidates for upscale synthesis to be used as an active drug molecule against breast cancer.
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Shekhar, R., Prakash, H. S., & Geetha, N. (2026). Phytofabrication of Magnetite Nanoparticles from Piper betle (L.) var. Mysore and Evaluation of Anti-Breast Cancer Potentials. Indian Journal of Pharmaceutical Education and Research, 60(2s), s533-s540. https://doi.org/10.5530/ijper.20261603
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